precision proteintm streptactin hrp conjugate (Bio-Rad)

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Bio-Rad precision proteintm streptactin hrp conjugate
Precision Proteintm Streptactin Hrp Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/precision+proteintm+streptactin+hrp+conjugate/Precision+Plus+Protein+Unstained+Protein+Standards/pmc13012864-51-12-20
Average 96 stars, based on 414 article reviews

precision proteintm streptactin hrp conjugate - by Bioz Stars, 2026-09

96/100 stars

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Article Title: Decoy Antibodies Block Extracellular HSP70, Prevent Self Signaling and Inhibit Melanoma Cell Survival
Article Snippet: Detection was performed using an HRP-conjugated anti-mouse IgG secondary antibody (Jackson ImmunoResearch), Precision ProteinTM StrepTactin-HRP Conjugate (1:5000, BioRad) and ECL reagent (Bio-Rad).

Saline:

Article Title: Polymethoxyflavones from Kaempferia parviflora stimulate melanogenesis by blocking the TPC2 channel.
Article Snippet: Bovine serum albumin, dimethyl sulfoxide, and RIPA lysis buffer were purchased from Merck Millipore (Billerica, MA, USA). .. Laemmli sample buffer (4×), Precision plus proteinTM westernCTM blotting standards, Precision proteinTM streptactin–HRP conjugate, 40% acrylamide, tetramethylethylenediamine, sodium dodecyl sulphate (SDS), resolving gel buffer, stacking gel buffer, polyvinylidene difluoride membranes, 10× Tris/Tricine/SDS buffer, 10× Tris/Glycine buffer, and 10× Tris buffered saline were purchased from Bio-Rad (Hercules, CA, USA). .. Tween 20 was purchased from LOBA (Chemie, Mumbai, India).

Article Title: Polymethoxyflavones from Kaempferia parviflora stimulate melanogenesis by blocking the TPC2 channel
Article Snippet: Bovine serum albumin, dimethyl sulfoxide, and RIPA lysis buffer were purchased from Merck Millipore (Billerica, MA, USA). .. Laemmli sample buffer (4×), Precision plus proteinTM westernCTM blotting standards, Precision proteinTM streptactin–HRP conjugate, 40% acrylamide, tetramethylethylenediamine, sodium dodecyl sulphate (SDS), resolving gel buffer, stacking gel buffer, polyvinylidene difluoride membranes, 10× Tris/Tricine/SDS buffer, 10× Tris/Glycine buffer, and 10× Tris buffered saline were purchased from Bio-Rad (Hercules, CA, USA). .. Tween 20 was purchased from LOBA (Chemie, Mumbai, India).

Incubation:

Article Title: Differential effects of KRAS G-domain and hypervariable region mutants on cancer phenotypes
Article Snippet: For the p-Akt activation blots, the following antibodies were used: rabbit anti-phospho-Akt (Ser473) (D9E) monoclonal antibody (Cat. #4060; Cell Signaling Technology; 1:500), rabbit anti-Akt (pan) (C67E7) monoclonal antibody (Cat. #4691; Cell Signaling Technology; 1:1000), rabbit anti-KRAS (11H35L14) monoclonal antibody (Cat. #703345; Invitrogen; 1:1000 dilution), and rabbit anti-GAPDH (14C10) monoclonal antibody (Cat. #2118; Cell Signaling Technology; 1:4000 dilution). .. A mixture of goat anti-rabbit IgG (H + L), HRP-conjugated (Cat. #31460; Invitrogen; 1:5000 dilution) and Precision ProteinTM StrepTactin-HRP conjugate (Bio-Rad Laboratories, Inc.; 1:10000) was used for secondary antibody incubation. .. Chemiluminescence images were acquired after addition of LuminataTM Classico Western HRP Substrate (Merck Millipore; Bulington, MA, USA).

Article Title: Differential effects of KRAS G-domain and hypervariable region mutants on cancer phenotypes.
Article Snippet: The following primary antibodies were used to detect KRAS overexpression and p-Erk: rabbit anti-K-Ras (11H35L14) monoclonal antibody (Cat. #703345; Invitrogen; 1:5000 dilution), rabbit anti-GAPDH (14C10) monoclonal antibody (Cat. #2118; Cell Signaling Technology; Danvers, MA, USA; 1:1000 dilution), and rabbit anti-phospho-p44/42 MAPK (Erk1/2) (Cat. #9101; Cell Signaling Technology; 1:1000 dilution). .. A mixture of goat anti-rabbit IgG (H + L), HRP-conjugated (Cat. #31460; Invitrogen; 1:2500 dilution) and Precision ProteinTM StrepTactin-HRP conjugate (Bio-Rad Laboratories, Inc.; 1:2500) was used for secondary antibody incubation. .. For the p-Akt activation blots, the following antibodies were used: rabbit anti-phospho-Akt (Ser473) (D9E) monoclonal antibody (Cat. #4060; Cell Signaling Technology; 1:500), rabbit anti-Akt (pan) (C67E7) monoclonal antibody (Cat. #4691; Cell Signaling Technology; 1:1000), rabbit anti-KRAS (11H35L14) monoclonal antibody (Cat. #703345; Invitrogen; 1:1000 dilution), and rabbit anti-GAPDH (14C10) monoclonal antibody (Cat. #2118; Cell Signaling Technology; 1:4000 dilution).

Article Title: Differential effects of KRAS G-domain and hypervariable region mutants on cancer phenotypes.
Article Snippet: For the p-Akt activation blots, the following antibodies were used: rabbit anti-phospho-Akt (Ser473) (D9E) monoclonal antibody (Cat. #4060; Cell Signaling Technology; 1:500), rabbit anti-Akt (pan) (C67E7) monoclonal antibody (Cat. #4691; Cell Signaling Technology; 1:1000), rabbit anti-KRAS (11H35L14) monoclonal antibody (Cat. #703345; Invitrogen; 1:1000 dilution), and rabbit anti-GAPDH (14C10) monoclonal antibody (Cat. #2118; Cell Signaling Technology; 1:4000 dilution). .. A mixture of goat anti-rabbit IgG (H + L), HRP-conjugated (Cat. #31460; Invitrogen; 1:5000 dilution) and Precision ProteinTM StrepTactin-HRP conjugate (Bio-Rad Laboratories, Inc.; 1:10000) was used for secondary antibody incubation. .. Chemiluminescence images were acquired after addition of LuminataTM Classico Western HRP Substrate (Merck Millipore; Bulington, MA, USA).

Article Title: Differential effects of KRAS G-domain and hypervariable region mutants on cancer phenotypes
Article Snippet: The following primary antibodies were used to detect KRAS overexpression and p-Erk: rabbit anti-K-Ras (11H35L14) monoclonal antibody (Cat. #703345; Invitrogen; 1:5000 dilution), rabbit anti-GAPDH (14C10) monoclonal antibody (Cat. #2118; Cell Signaling Technology; Danvers, MA, USA; 1:1000 dilution), and rabbit anti-phospho-p44/42 MAPK (Erk1/2) (Cat. #9101; Cell Signaling Technology; 1:1000 dilution). .. A mixture of goat anti-rabbit IgG (H + L), HRP-conjugated (Cat. #31460; Invitrogen; 1:2500 dilution) and Precision ProteinTM StrepTactin-HRP conjugate (Bio-Rad Laboratories, Inc.; 1:2500) was used for secondary antibody incubation. .. For the p-Akt activation blots, the following antibodies were used: rabbit anti-phospho-Akt (Ser473) (D9E) monoclonal antibody (Cat. #4060; Cell Signaling Technology; 1:500), rabbit anti-Akt (pan) (C67E7) monoclonal antibody (Cat. #4691; Cell Signaling Technology; 1:1000), rabbit anti-KRAS (11H35L14) monoclonal antibody (Cat. #703345; Invitrogen; 1:1000 dilution), and rabbit anti-GAPDH (14C10) monoclonal antibody (Cat. #2118; Cell Signaling Technology; 1:4000 dilution).

Western Blot:

Article Title: Berry e-liquid flavour toxicities are not equal on an alveolar-capillary barrier cell model according to two exposure methods
Article Snippet: Normal goat serum (#S2000-100) was obtained from Dutscher (Bernolsheim, France). .. Western blot membranes (#1704157), Precision Plus ProteinTM Unstained Protein Standards (#1610363) and Precision ProteinTM StrepTactin-HRP Conjugate (#161-0,380) were acquired at Bio-rad (Hercule, United States). .. Tris Glycine-SDS Buffer 10X (#EU0510) was obtained from Euromedex (Souffelweyersheim, France).

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